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ULTRAWEAK PHOTON EMISSION IN ASSESSING GROWTH FACTOR EFFICIENCY USING FIBROBLASTIC DIFFERENTIATION

Hugo J. Niggli 1,2,3*

1 BioFoton AG, Rte. d'Essert 27, CH-1733 Treyvaux, Switzerland.Phone/fax: 0041264131445

2 Department of Obstetrics, Laboratory of Oxidative Stress and Aging, University Hospital (CHUV), CH-1011 Lausanne, Switzerland

3 International Institute of Biophysics IIB, ehemalige Raketenstation, Kapellener Strasse, D-41472 Neuss, Germany

* Address for correspondence: BioFoton AG, P.O. Box 28, CH-1731 Ependes, Switzerland

E-Mail: biofoton@swissonline.ch

Key words: Ultraweak, photon, biophoton, fibroblast skin, human ultraviolet, growth factor

Abstract

Photons participate in many atomic and molecular interactions and changes. Recent biophysical research has shown the existence of ultraweak photons (biophotons) in biological tissue 1,2,3. Recently biochemical investigations sustain the hypotheis that pyrimidine dimer formation and excision seems to be involved in a type of intra- and extracellular photobiostimulation and may be important triggers of UV-induced signal pathways expressing epidermal communciation 4. Although the emission is extremely low in mammalian cells, it can be readily detected with sophisticated photomultiplier systems and experiments with cultured human cells have shown that biophotons can be efficiently induced by ultraviolet light. Photons in the visible range are coupled with radical reactions while photons in the UV are link with the DNA as source.

In the experiments shown here, bone growth factors were tested on their growth stimulation efficiency in quantities as low as 5 ng, using the differentiation system of human skin fibroblasts with and without irradiation of UVA and UVB. The different batches of growth factors showed various proliferation of skin fibroblasts in culture which could be correlated with the biophoton emission. The growth factors reduced the acceleration of the fibroblast differentiation induced by a chemical agent (Mitomycin C) by a factor of 10-30%. In view that fibroblasts play an essential role in skin aging and wound healing, the fibroblastic differentiation is a powerful tool in order to eluciate basic processes in intra- and extracellular biocommunication.

References

(1) Tilbury, R.N., Slawinski, J. , Ezzahir,A. , Godlewski, M., Kwiecinska, T. ,Rajfur, Z., Sitko, D., Wienzuchowska, D. ,Kochel, B., Gu, Q., Popp , F.A.,Lilius, E.M., Marnila, P., Van Wijk R., and J.M. Van Aken, Multi-author review on Biophoton emission, stress and disease, Experientia 48 (1992) 1029-1102.

(2) Popp, F.A.. (1999) Die Botschaft der Nahrung. Verlag, 2001. D-60381 Frankfurt.

(3) Niggli, H.J. (1993), Artificial sunlight irradiation induces ultraweak photon emission in human skin fibroblasts, J. Photochem. Photobiol. 18, 281-285.

(4) Applegate, L. A. , Scaletta, C. Panizzon, R. Niggli, H. and E. Frenk: In vivo induction of pyrimidine dimers in human skin by UVA radiation: Initiation of cell damage and/or intercellular communication. Intern. J. of Molecular Med. 3 (1999) 467-472..

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